This product is a recombinant Mouse antibody that recognizes Mucin1. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Figure 3 Anti-Human MUC1 Antibody (TAB-428MZ) in Dot Blot
Dot Blot analysis of TAB-428MZ was performed by coating with Recombinant Human MUC1 Protein (his Tag).
TAB-428MZ incubation concentration: 1 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Figure 1 Anti-Human MUC1 Antibody (TAB-428MZ) in ELISA
ELISA analysis of TAB-428MZ was performed by coating with Recombinant Human MUC1 Protein (his Tag). Then blocked with BSA and incubated with Anti-Human MUC1 Antibody (TAB-428MZ). The HRP-conjugated Goat Anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-Human MUC1 Antibody (TAB-428MZ) in WB
Western blot analysis of TAB-428MZ was performed by loading Recombinant Human MUC1 Protein (his Tag) onto a 12% Tris-HCl polyacrylamide gel. Membranes were probed with TAB-428MZ and HRP Goat Anti-Mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
Left: Reduced. Right: Non-reduced
Lane 1: 0.2 μg
Lane 2: 0.4 μg
Lane 3: 0.8 μg
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-428MZ, RRID: AB_3111941)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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